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Norgen Biotek™ FFPE RNA Purification Spin Column Kit, 50 Preps
Norgen Biotek Corp.™
$435.00
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Kit Specifications
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| Maximum Column Binding Capacity |
Up to 50 µg RNA
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| Maximum Loading Volume Per Spin Column |
650 µL
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| Size of RNA Purified |
All sizes, including small RNA (< 200 nt)
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| Time to Complete 10 Purifications |
1-4 hours*
|
| Maximum Amount of Starting Material |
5 slices of < 20 µm thick paraffin slices
25 mg of unsectioned block |
| Average Yield |
Variable due to age of paraffin blocks
~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney |
* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.
| Component | Cat. 25300 (50 preps) | Cat. 25400 (2 x 96 preps) |
|---|---|---|
| Digestion Buffer A | 25 mL | 2 x 25 mL |
| Buffer RL | 30 mL | 2 x 30 mL |
| Enzyme Incubation Buffer | 6 mL | 2 x 6 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 20 mL |
| Proteinase K | 12 mg | 2 x 20 mg |
| DNase I | 1 vial | 2 x 500μL |
| Micro Spin Columns | 50 | - |
| 96-Well Incubation Plate | - | 2 |
| 96-Well Plate | - | 2 |
| Adhesive Tape | - | 8 |
| Collection Tubes | 50 | - |
| 96-Well Collection Plate | - | 2 |
| Elution Tubes (1.7 mL) | 50 | - |
| 96-Well Elution Plate | - | 2 |
| Product Insert | 1 | 1 |
