Norgen Biotek™ HighRanger 1kb DNA Ladder, 5 x 100 Loads
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HighRanger Plus 100 bp DNA Ladder (Cat# 12000) - 100 loads
Ladder Properties:
- Nineteen discrete fragments ranging from 100 bp to 10000 bp
- Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference
Fragment
|
Size (bp)
|
Mass (ng)
|
1
|
10000
|
42
|
2
|
8000
|
33
|
3
|
6000
|
25
|
4
|
5000
|
42
|
5
|
4000
|
35
|
6
|
3000
|
27
|
7
|
2500
|
22
|
8
|
2000
|
18
|
9
|
1500
|
13
|
10
|
1000
|
40
|
11
|
900
|
28
|
12 | 800 |
25 |
13 | 700 |
34 |
14 | 600 |
19 |
15 | 500 |
44 |
16 | 400 |
13 |
17 | 300 |
18 |
18 | 200 |
13 |
19 | 100 |
10 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.