SKU: 56100
Norgen Biotek™ Plasma/Serum RNA Purification Midi Kit, 20 Preps
Norgen Biotek Corp.™
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Kit Specifications
|
|
Minimum Plasma/Serum Input
|
2 mL
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Maximum Plasma/Serum Input
|
5 mL
|
Size of DNA Purified |
All sizes, including miRNA
and small RNA (<200 nt) |
Elution Volume |
50-100 μL
|
Time to Complete 10 Purifications |
35-40 minutes
|
Average Yields* |
Variable depending on specimen
|
*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.
This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Kit Components | Cat. 55000 (50 preps) | Cat. 56100 (20 preps) | Cat. 56200 (10 preps) |
---|---|---|---|
Lysis Buffer A | 2 x 20 mL | 100 mL | 1 x 130 mL 1 x 30 mL |
Wash Solution A | 18 mL | 1 x 38 mL 1 x 18 mL |
38 mL |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Elution Buffer F | - | 15 mL | 15 mL |
Micro Spin Columns | 50 | - | - |
Mini Spin Columns | - | 20 | 10 |
Midi Spin Columns | - | 20 | - |
Maxi Spin Columns | - | - | 10 |
Collection Tubes | 50 | 20 | 10 |
Elution Tubes (1.7 mL) | 50 | 20 | 10 |
Product Insert | 1 | 1 | 1 |