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Norgen Biotek™ ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit, 4 Preps

SKU:
17700
$179.00
Net 30 Terms Available

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All-in-one solution for inclusion body protein isolation and purification Fast and convenient spin column protocol Complete kit with Cell Lysis Reagent, Inclusion Body Solubilization Reagent, buffers and spin columns to purify proteins Purification is based on spin column chromatography that uses Norgen’s resin separation matrix This ki…

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Kit Specifications
Maximum Culture Volume
100 mL
Yield from 100 mL of Culture
Up to 12 mg
Minimum Elution Volume
4 mL
Time to Process 1 Sample
1 hour

Storage Conditions
The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for Binding Buffer C and Binding Buffer N. Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.

Component Cat. 10300 (Micro - 25 preps) Cat. 17700 (Maxi - 4 preps)
Wash Solution C 30 mL 130 mL
Wash Solution N 30 mL 130 mL
Binding BUffer A 4 mL 20 mL
Binding Buffer N 4 mL 20 mL
Elution Buffer C 8 mL 2 x 30 mL
Protein Neutralizer 4 mL 4 mL
Cell Lysis Reagent 15 mL 110 mL
IB Solubilization Reagent 2 mL 50 mL
Syringes, 1cc, slip tip 25 -
Needles (Bev, 20G x 1 inch) 25 -
Syringes, 10 mL, Luer-Lok™ Tip - 4
Needles (18G x 1.5 inch) - 4
Micro Spin Columns 25 -
Maxi Spin Columns (filled with SiC) inserted into 50 mL collection tubes - 4
Collection Tubes 25 -
Elution Tubes (1.7 mL) 25 -
Elution Tubes (50 mL) - 4
Product Insert 1 1
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