SKU: 30300
Norgen Biotek™ Detergent-Free Total Protein Isolation Kit, 25 Preps
$169.00
Norgen Biotek Corp.™
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Kit Specifications
|
|
Maximum Protein Input |
4 mg
|
Minimum Protein Input |
0.25 mg
|
Maximum Column Volume Input | 18 mL |
Molecular Weight of Recovered Proteins | No Molecular Weight cut-off |
Final Endotoxin Levels |
≤ 0.01 EU/μg protein
|
Protein Recovery |
90-95%
|
% Detergent Removal | 90-95% |
Detergents that can be Removed | Including Triton® X-100, CHAPS, NP-40 and Tween 20 |
Elution Volume | 2-4 mL |
Time to Complete 10 Purifications |
30-40 minutes
|
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 22800 (25 preps) | Cat. 22200 (4 preps) |
---|---|---|
Binding Buffer J | 8 mL | 8 mL |
Binding Buffer N | 4 mL | 20 mL |
Wash Solution M | 50 mL | 130 mL |
Wash Solution CIP | 20 mL | 60 mL |
Wash Solution N | 30 mL | 130 mL |
Wash Solution NIP | 20 mL | 60 mL |
Elution Buffer G | 6 mL | 20 mL |
Endotoxin Removal Solution | 1.5 mL | 1.5 mL |
Protein Neutralizer EF | 2 mL | 2 mL |
Maxi Spin Columns (assembled with Collection Tubes) | - | 4 |
Mini Spin Columns | 25 | - |
Collection Tubes | 25 | - |
Maxi Spin Columns (assembled with Collection Tubes) | 4 | |
Elution Tubes (1.7 mL) | 25 | - |
Elution Tubes (50 mL) | 4 | - |
Product Insert | 1 | 1 |