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Norgen Biotek™ RNA/DNA/Protein Purification Plus Micro Spin Column Kit, 50 Preps
Norgen Biotek Corp.™
$799.00
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Kit Specifications
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Maximum Column Binding Capacity
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35 μg for RNA
10 μg for DNA 100 μg for protein |
| Maximum Column Loading Volume |
650 μL
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Size of RNA Purified
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All sizes, including
small RNA (< 200 nt) |
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Size of DNA Purified
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≥ 30 kb
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| Time to Complete 10 Purifications |
30 minutes
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Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Blood
Plant / Fungi Tissues
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5 x 105 cells
5 mg (for selected tissues)
50 µL
25 mg
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| Average Yield* HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells) |
RNA: 7.5 μg DNA: 1-2 μg Protein: 70 μg |
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 47700 (50 preps) | Cat. 51600 (50 preps) | Cat. 51700 (96 preps) |
|---|---|---|---|
| Buffer SKP | 40 mL | 40 mL | - |
| Lysis Buffer Q | - | - | 40 mL |
| Wash Solution A | 2 x 38 mL 1 x 18 mL |
2 x 38 mL 1 x 18 mL |
2 x 38 mL 1 x 18 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL |
| Elution Buffer F | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution C | 30 mL | 30 mL | 60 mL |
| Binding Buffer A | 8 mL | 8 mL | 8 mL |
| Elution Buffer C | 8 mL | 8 mL | 30 mL |
| Protein Neutralizer | 4 mL | 4 mL | 4 mL |
| Protein Loading Dye | 2 mL | 2 mL | 3 x 2 mL |
| gDNA Purification Columns | 50 | - | - |
| gDNA Purification Micro Columns | - | 50 | - |
| gDNA Purification 96-Well Plate | - | - | 1 |
| RNA/Protein Purification Columns | 50 | - | - |
| RNA/Protein Purification Micro Columns | - | 50 | - |
| RNA/Protein Purification 96-Well Plate | - | - | 1 |
| Collection Tubes | 150 | 150 | - |
| Collection Plate | - | - | 5 |
| Elution Tubes (1.7 mL) | 150 | 150 | - |
| Elution Plate | - | - | 3 |
| Lysis Preparation Plate | - | - | 2 |
| Adhesive Tape | - | - | 4 |
| Product Insert | 1 | 1 | 1 |
