Norgen Biotek™ Small RNA Library Prep Kit for Illumina (Indexes 1-24), 24 Preps

Norgen Biotek Corp.™

SKU:

63600

$1,635.00

Net 30 Terms Available

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Optimized for low input RNA, especially from bodily fluids such as plasma or serum at 1ng of RNA Simple and quick workflow: library could be prepared in less than 5 hours No gel purification for selected types of samples Protocol optimized for RNA isolated from different types of input, including liquid biopsies (blood, plasma, serum and urine)…

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Kit Specifications
Number of preps	24
Number of indices provided	12 (for 24 preps, indexes 1-24 or 25-48)
Time required to complete 6 libraries	As little as 5 hours
Input RNA required	As little as 50 pg

Storage Conditions and Product Stability
Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.

Step	Component	Cat. 64600 (24 preps)
3' AdaptorLigation to Template RNA	3' Adaptor	30 µL
	3' Adaptor Ligation Master Mix	320 µL
	T4 RNA Ligase 2 (Truncated)	35 µL
5' Adaptor Ligation	5' Adaptor	30 µL
	5' Adaptor Ligation Master Mix	320 µL
	T4 RNA Ligase 1	35 µL
cDNA Synthesis from Ligated RNA Product	Reverse Primer	30 µL
	cDNA Synthesis Master Mix	220 µL
	TruScript ReverseTranscriptase	35 µL
PCR Amplification	2x NGS PCR Master Mix	1.32 µL
	PCR Reverse Primer	81 µL
	Forward Index Primer	Included in Small RNA Library Prep Forward Index Primers (# 64640 or # 64610)
Size Selection	NGS MW Ladder	50 µL
	NGS Control Ladder	50 µL
	Loading Dye	300 µL
	Nuclease-Free Water	1.25 µL

Component	Cat. 63500 (75 preps)
Buffer RL	40 mL
Wash Solution A	38 mL (Reconstituted to 128 mL)
Elution Solution A	6 mL
Columns	75
Gel Filtration Columns	24
Collection Tubes	75
Elution Tubes	75
Product Insert	1