Norgen Biotek™ Total RNA Purification Kit - 96 Deep Well Plate Format Dx, 2 Plates

Norgen Biotek Corp.™

SKU:

Dx24350

$825.00

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CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746. Available for sale in Canada (Dx17200, Dx24300) Ideal for use in in vitro diagnostic workflows Extract high quality & quantity total RNA including miRNA No phenol step required Bind & elute all RNA irrespective of size or GC content, withou…

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Available for sale in Canada (Dx17200, Dx24300)
Ideal for use in in vitro diagnostic workflows
Extract high quality & quantity total RNA including miRNA
No phenol step required
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Convenient & fast spin column format
Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Kit Dx (Dx24350) provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)

Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Intended Use

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

NOTE: This product is not available for sale in the United States.

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 10⁶ Cells) E. coli (1 x 10⁹ cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL 150 µL 1 x 10⁹ cells 1 x 10⁸cells 40 mg 40 mg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. Dx17200 (50 preps)	Cat. Dx24300 (192 preps)	Cat. Dx24350 (192 preps)	Cat. Dx24380 (576 preps)
Lysis Solution	40 mL	-	-	-
Buffer RL	-	2 x 40 mL	2 x 40 mL	350 mL
Wash Solution	22 mL	-	-	-
Wash Solution A	22 mL	2 x 38 mL	2 x 38 mL	1 x 148 mL 1 x 74 mL
Elution Solution	6 mL	-	-	-
Elution Solution A	-	2 x 20 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	-	-	-
96-Well Isolation Plate	-	2	2	-
96-Well Isolation Plate (Deep Well)	-	-	-	6
Collection Tubes	50	-	-	-
96-Well Collection Plate	-	2	2	-
96-Well Collection Plate (Deep Well)	-	-	-	6
Elution Tubes (1.7 mL)	50	-	-	-
96-Well Elution Plate	-	2	2	-
96-Well Elution Plate (Deep Well)	-	-	-	6
Adhesive Tape	-	4	4	12
Product Insert	1	1	1	1

Protocol

Supplementary Protocol

Application Note

Poster

Safety Data Sheet

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