SKU: 17270
Norgen Biotek™ Total RNA Purification Kit Spin Column, 500 Preps
Norgen Biotek Corp.™
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Kit Specifications
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Maximum Binding Capacity
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Up to 50 μg RNA
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Maximum Loading Volume
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650 μL
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Size of RNA Purified
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All sizes, including small RNA (< 200 nt)
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Maximum Amount of Starting Material
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Animal Cells | 3 x 106 cells |
Animal Tissues | 10 mg (for most tissues*) |
Blood | 100 μL |
Plasma/Serum | 200 μL |
Bacteria | 1 x 109 cells |
Yeast |
1 x 108 cells
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Fungi |
50 mg
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Plant Tissues |
50 mg
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Time to Complete 10 Purifications |
20 minutes
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Average Yield | |
HeLa Cells (1 x 106 cells) | 15 μg |
E. coli (1 x 109 cells) | 50 μg |
* for isolating total RNA from larger amounts of tissue, please use Norgen's Animal Tissue RNA Purification Kit (Cat# 25700)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) | Cat. 17250 (250 preps) | Cat. 17270 (500 preps) | Cat. 24300 (192 preps) | Cat. 24370 (576 preps) | Cat. 24350 (192 preps) | Cat. 24380 (576 preps) |
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Buffer RL | 40 mL | 2 x 40 mL | 175 mL | 350 mL | 2 x 40 mL | 350 mL | 2 x 40 mL | 350 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 148 mL | 1 x 148 mL 1 x 74 mL |
2 x 38 mL | 1 x 74 mL 1 x 148 mL |
2 x 38 mL | 1 x 74 mL 1 x 148 mL |
Elution Solution A | 6 mL | 2 x 6 mL | 30 mL | 60 mL | 2 x 20 mL | 60 mL | 2 x 20 mL | 60 mL |
Mini Spin Columns | 50 | 100 | 250 | 500 | - | - | - | - |
96-Well Isolation Plate | - | - | - | - | 2 | 6 | - | - |
96-Well Isolation Plate (Deep Well) | - | - | - | - | - | - | 2 | 6 |
Adhesive Tape | - | - | - | - | 4 | 12 | 4 | 12 |
Collection Tubes | 50 | 100 | 250 | 500 | - | - | ||
96-Well Collection Plate | - | - | - | - | 2 | 6 | - | - |
96-Well Collection Plate (Deep Well) | - | - | - | - | - | - | 2 | 6 |
Elution Tubes (1.7 mL) | 50 | 100 | 250 | 500 | - | - | ||
96-Well Elution Plate | - | - | - | - | 2 | 6 | - | - |
96-Well Elution Plate (Deep Well) | - | - | - | - | - | - | 2 | 6 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |