Norgen Biotek™ Total RNA Purification Micro Kit, 50 Preps
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Kit Specifications
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Maximum Column Binding Capacity
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35 μg
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Maximum Column Loading Volume
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650 μL
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| Minimum Elution Volume |
20 μL
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| Size of RNA Purified |
All sizes, including small RNA
(< 200 nt) |
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Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM) |
5 x 105 cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 105 cells |
| Time to Complete 10 Purifications |
20 minutes
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Average Yield
HeLa Cells (1 x 105 cells) |
~1.5 μg |
* for fibrous tissue, an additional Proteinase K treatment is required
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 35300 (50 preps) | Cat. 35350 (250 preps) |
|---|---|---|
| Buffer RL | 40 mL | 5 x 40 mL |
| Wash Solution A | 38 mL | 5 x 38 mL |
| Elution Solution A | 6 mL | 5 x 6 mL |
| Micro Spin Columns | 50 | 250 |
| Collection Tubes | 50 | 250 |
| Elution Tubes (1.7 mL) | 50 | 250 |
| Product Insert | 1 | 1 |