pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb, Polyclonal (A20303) | ABclonal Technology™

ABclonal Technology™ pan-Ubiquitin Remnant Motif (K-ε-GG) Rabbit pAb (A20303) is a polyclonal antibody raised in rabbit. It is validated for WB and ELISA. Confirmed reactivity includes Human, Mouse and Rat. The antibody has an IgG isotype and an observed molecular weight of >10kDa/＞10kDa. It is affinity purified.

Ubiquitination plays a key role in protein degradation and signal transduction. Characterization of ubiquitination sites is important for understanding the role of this modification in cellular processes and disease. Ubiquitination sites are usually identified by detection of Lys-?-Gly-Gly (K-?-GG)-remnant peptides, which are generated by tryptic digestion of proteomes. The di-glycine remnant left at sites of ubiquitination after trypsin digestion through cleavage of the C-terminal –RGG sequence on ubiquitin (K-ε-GG). This Ubiquitin K-ε-GG remnant motif antibody will recognize the di-glycine remnant independent of flanking amino acid sequence.

• Host Species: Rabbit
• Isotype: IgG
• Species Reactivity: Human, Mouse, Rat
• Validated Applications: WB, ELISA
• Recommended Dilution: WB,1:500 - 1:1000; ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Purity: Affinity purification
• Observed MW: >10kDa/＞10kDa
• Storage: Store at -20℃. Avoid freeze / thaw cycles.; Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

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