PANSORBIN® Cells, 10 g (507858-10GM)

Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes. Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2. Kierszenbaum, F., et al. 1991. Immunology74, 317. Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569. Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368. Murakami, H., et al. 1988. Biochem. J.256, 917. Kessler, S.W. 1975. J. Immunol. 115, 1617. Toxicity: Standard Handling (A)

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