Protein A, Biotin Conjugate, 1 X 2 mg (203195-2MG)

Native protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species. Solid Phase Immunoassay (1:10,000) In 10 mM PBS, 1% BSA, pH 7.6. The protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution. A single band at MW 50,000 by SDS-PAGE Following initial thaw, aliquot and freeze (-20°C). Toxicity: Standard Handling (A)

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