Protein Molecular Weight Markers, HPLC, 5 tests (539053-5T)

Contains five highly purified proteins ranging in molecular weight from 12.4 kDa to 290 kDa. Formulated to give peaks of approximately the same intensity for each protein when run on a TSK-Gel G300SW column after reconstituting with elution buffer and monitored at 280 nm. Markers include: cytochrome c, 12,400; myokinase, 32,000; enolase, 67,000; lactate dehydrogenase, 142,000; and glutamate dehydrogenase, 290,000. Reconstitute with 100 µl of 100 mM sodium phosphate buffer, pH 7.0 containing 200 mM NaCl. If the material is reconstituted with lower ionic strength buffer (i.e., <0.1 M NaCl) the cytochrome c may interact nonspecifically with the silica gel column and migrate incorrectly. Apply 20 µl to an analytical grade column such as TSK-Gel G3000SW (0.75 x 60 cm). Toxicity: Standard Handling (A)

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