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Radiant™ GC-Long Hot-Start 2X Master Mix


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Radiant GC-Long Hot Start 2x Master Mix has been engineered for the amplification of extremely difficult templates and long amplicons up to 35kb. Radiant GC-Long Hot Start 2x Master Mix possesses 5´-3´ DNA polymerase and 3´-5 proofreading exonuclease activities with an error-rate of 4.55 x 10-7 .

The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences. Radiant GC-Long Hot Start 2x Master Mix Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase. Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples.

  • >50-fold higher fidelity than standard Taq DNA Polymerase
  • New-generation PCR buffer formulation for maximum PCR efficiency and reaction speed.
  • Robust PCR performance across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences.
  • Supplied as a 2X Master Mix containing MgCl2 and highly pure dNTPS for maximum convenience and minimal liquid handling.
  • High DNA yields with either fast cycling or standard cycling.
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