Radiant™ HiFi Polymerase

Radiant HiFi Polymerase is a high-performance, high-fidelity DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA.

Radiant HiFi Ultra Polymerase possesses 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities with an error-rate of 4.55 x 10-7 . The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM HiFi Ultra Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase.

Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples. The supplied 5X HiFi Ultra Reaction Buffer contains an ideal concentration of MgCl2 and highly pure dNTPS for convenience and consistency in liquid handling.

• >50-fold higher fidelity than standard Taq DNA Polymerase
• New-generation PCR buffer formulation for maximum PCR efficiency and reaction speed.
• Robust PCR performance across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences.
• Supplied with 5X HiFi Ultra Reaction Buffer containing MgCl2 and highly pure dNTPS.
• High DNA yields with either fast cycling or standard cycling.

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