Recombinant Human Carbonic anhydrase 5A/CA5A Protein (RP01791) | ABclonal Technology™

ABclonal Technology™ Recombinant Human Carbonic anhydrase 5A/CA5A Protein (RP01791) is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala40-Ser305) of human CA5A (Accession #NP_001730.1) fused with 6×His tag at the C-terminus. Purity: ≥ 90 % as determined by SDS-PAGE. Each lot is quality controlled to support reproducible results in research applications.

Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VA encoded by the CA5A gene is a mitochondrial protein (2, 3). In comparison with another mitochondrial CA (CA5B), CA5A has different tissue distribution and chromosomal location (4, 5). Expression and inhibitor studies of different CAs in the rat pancreatic beta cells indicate that CA5A may be involved in the regulation of insulin secretion (6). CA5A may also participate in the detoxification of ammonia produced in the gastrointestinal tract by providing bicarbonate to carbamyl phosphate synthetase I (7). The amino acid sequence of recombinant human CA5A (residues 40 to 305) is 79%, 77%, and 76% identical to that of canine, bovine, and rat/mouse.

• Species: Human
• Expression System: HEK293 cells
• Tag: C-His
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Endotoxin: < 0.01 EU/μg of the protein by LAL method
• Bioactivity: Measured by its esterase activity. The specific activity is >600 pmol/min/µg, as measured under the described conditions.
• UniProt ID: P35218
• Formulation: Lyophilized from a 0.22 μm filtered solution of 50mM NaAc,50mM NaCl,0.05% Brij-35，pH 5.0.
• Storage: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.; After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.

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