Recombinant Human HGF Activator/HGFA Protein (RP03236) | ABclonal Technology™

ABclonal Technology™ Recombinant Human HGF Activator/HGFA Protein (RP03236) is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln36-Ser655) of Human HGF Activator/HGFA (Accession #NP_001519.1) fused with His tag at the C-terminus. Purity: ≥ 95 % as determined by SDS-PAGE. Each lot is quality controlled to support reproducible results in research applications.

HGF activator (HGFA) is a serum-derived serine protease and belongs to the peptidase family S1.HGFA is responsible for the conversion of hepatocyte growth factor (HGF), from the inactive single-chain precursor to the active heterodimeric form, which is a potent mitogen, motogen, and morphogen for liver cells, epithelial cells, and endothelial cells. HGFA is synthesized and secreted by the liver and circulates in the plasma as an inactive single-chain zymogen in normal states. The zymogen is cleaved by thrombin or thermolysin through the endoproteolytic process and forms an active heterodimer linked by a disulfide bond. In turn, the active protease can be inhibited by HGFA inhibitors (HAIs) including HAI-1 and HAI-2. Besides, the HGFA zymogen acquires a strong affinity upon activation and thus may ensure the local action in tissue regeneration in the liver, kidney, and skin. It has been reported that activation of HGF is a critical limiting step in the HGF/SF-induced signaling pathway mediated by Met, and accordingly, aberrant expression of HGFA is implicated in tumorigenesis and progression.

• Species: Human
• Expression System: HEK293 cells
• Tag: C-6His
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Endotoxin: < 0.01 EU/μg of the protein by LAL method
• UniProt ID: Q04756
• Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Storage: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.; After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.

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