Recombinant Mouse Carbonic Anhydrase IX/CA9 Protein (RP01495) | ABclonal Technology™

ABclonal Technology™ Recombinant Mouse Carbonic Anhydrase IX/CA9 Protein (RP01495) is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Asp390) of mouse Carbonic Anhydrase IX/CA9 (Accession #NP_647466.2) fused with a 6×His tag at the C-terminus. Purity: ≥ 95 % as determined by SDS-PAGE. Each lot is quality controlled to support reproducible results in research applications.

Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

• Species: Mouse
• Expression System: HEK293 cells
• Tag: C-His
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Bioactivity: Measured by its esterase activity. The specific activity is >750 pmol/min/µg, as measured under the described conditions.
• UniProt ID: Q8VHB5
• Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Storage: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.; After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.

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