Restorase® DNA Polymerase with 10× Reaction Buffer, 1 X 50 reactions (R1028-50RXN)

Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates. Restorase® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair. Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes. DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage. •Reliable amplification of damaged DNA •Increase Yield •Can repair 3′ bungs, nicks, and abasic sites

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