Ribonuclease A from bovine pancreas, 1 X 50 mg (R4642-50MG)

RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. Suitable for: •RNase protection assays •Removal of unspecifically bound RNA •Analysis of RNA sequences •Hydrolysis of RNA contained in protein samples •Plasmid DNA purification Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119. Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification. Protein determined by E. RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0). A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

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