RIPA Buffer with EDTA + EGTA

Premixed ready-to-use liquid. Contains 50mM TRIS-HCl (pH 7.4), 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS with 5mM EDTA and 1mM EGTA. Used in Radio-Immunoprecipitation Assay (RIPA), for cell lysis and protein solubilization. EDTA and EGTA act as metal chelators for Mg ions and Ca ions respectively.