Sorensen Buffer 500mL Bottle , 6/CS

Sorensen Buffer is a classic phosphate buffer system widely used in histology, hematology, and general biology to maintain a stable pH environment. This case of six 500mL bottles provides a reliable stock of standardized buffer, eliminating the variability often found when using tap water or manually mixed solutions.

The "Staining" Standard (Hematology): While used in many applications, Sorensen Buffer is most critical in the preparation of blood smears using Wright’s or Giemsa stains.

1. Color Consistency: The pH of the water used to rinse a blood slide dictates the color of the cells. If the water is too acidic, red blood cells look too pink and white blood cell nuclei become pale. If too alkaline, everything looks blue/black.

2. The "Sorensen" Fix: This buffer locks the pH (typically around pH 6.8 or 7.2, depending on the specific formulation) to the optimal point for the "Romanowsky" effect. This ensures that nuclear chromatin stains a distinct purple and erythrocytes stain a proper pink-orange, allowing pathologists to accurately identify cell types and anomalies.

Other Key Applications:

• Electron Microscopy (EM): Frequently used as a vehicle for fixatives (like Glutaraldehyde or Osmium Tetroxide) because the phosphate concentration helps maintain cellular structure and osmotic balance during fixation.

• Enzymatic Assays: Provides a stable, neutral environment for biochemical reactions that are sensitive to pH fluctuations.

• Tissue Processing: Used to wash tissue samples without causing lysis or cell distortion.

The Case Format (6/CS):

• Lab Standard: The case of 6 is ideal for high-volume histology or pathology labs that run daily staining racks.

• Benchtop Ready: The 500mL size is manageable for daily use on the workbench, unlike heavy carboys.

• pH Stabilizer: Resists changes in acidity/alkalinity to protect samples.

• Staining Quality: Essential for consistent Wright-Giemsa results in hematology.

• Cell Protection: Maintains osmotic balance to prevent cell lysis during washing.

• Versatile: Used in histology, EM, and general biochemistry.

• Format: Six 500mL bottles per case.

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