SPINeasy 96-Well DNA Kit for Tissue

The SPINeasy® 96-Well DNA Kit for Tissue is a high-performance tissue genomic DNA (gDNA) extraction kit in a 96-well plate format. The DNA purification protocol does not require any organic solvent or sophisticated equipment. Our specially formulated Buffer TD1 and Proteinase K provide effective lysis of various types of samples including tough, fibrous, and fatty tissues. Furthermore, the subsequent addition of Buffer TD2 enables selective binding of DNA to 96 DNA Plate M. The gDNA extracted from multiple types of tissues using this kit showed optimal integrity and purity which can immediately be used for most downstream applications, including long fragment PCR, real time PCR, restriction enzyme digestion and sequencing. Kit performance Figure 1: Comparison of quality and quantity of gDNA extracted from various sample types using SPINeasy® 96-Well DNA Kit for Tissue and Competitor Kit O and Kit Z. The values of yield, A260/A230 and A260/A280 ratio (from 15 mg of respective tissues) were measured with a spectrometer. The virtual gel image and DNA Integrity Number (DIN, in green or yellow) were analyzed using Agilent Tapestation 4150. Figure 2: Amplifiability of DNA extracted with SPINeasy® 96-Well DNA Kit for TissueUpper panel: Agarose gel electrophoresis of PCR products amplified from gDNA isolated from various mouse tissues using SPINeasy® 96-Well DNA Kit for Tissue. 1 mL of extracted gDNA was amplified using mouse-specific α-actin primers (1 kb product). Reactions without DNA (Sterile water) served as negative controls (NC).  Lower panel: Comparison of threshold cycles (Ct) of qPCR when amplification was performed using equal quantity of fat, kidney, lung, muscle, and tail gDNA (~25 ng) extracted with SPINeasy® 96-Well DNA Kit for Tissue and Competitor Kit O and Kit Z.  Reactions without DNA (Sterile water) served as negative controls (NC).

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