SPINeasy 96-Well Plasmid Miniprep Kit

SPINeasy 96-Well Plasmid Miniprep Kit enables a simple and high-throughput purification of plasmid DNA from bacterial hosts. This kit employs a silica-membrane spin plate in conjunction with an Alkaline Lysis procedure. It effectively captures plasmid DNA while swiftly eliminating undesired proteins and contaminants, completing the process in as little as 45 minutes. The highly purified plasmid DNA extracted with this kit is immediately ready for routine molecular biology laboratory applications such as endpoint PCR and restriction enzyme digestion. Following are the results of purification of plasmid DNA from SPINeasy 96-Well Plasmid Miniprep Kit and competitor T (in 3 replicates) using either Centrifugation Protocol (figure 1) or Vacuum Protocol (figure 2). Plasmid DNA purified with SPINeasy 96-Well Plasmid Miniprep Kit were of high purity, and the yield were much higher than that of competitor T. Figure 1. Agarose gel electrophoresis of plasmid DNA purified with SPINeasy 96-Well Plasmid Miniprep Kit (Lane 1-3) and Competitor T (Lane 4-6), using Centrifugation Protocol. Figure 2. Agarose gel electrophoresis of plasmid DNA purified with SPINeasy 96-Well Plasmid Miniprep Kit (Lane 1-3) and Competitor T (Lane 4-6), using Vacuum Protocol. Plasmid DNA purified using SPINeasy 96-Well Plasmid Miniprep Kit is also suitable for restriction enzyme digestion and endpoint PCR. Figure 3. Plasmid DNA purified using SPINeasy 96-Well Plasmid Miniprep Kit can directly be used for restriction enzyme digestion and endpoint PCR. M: DNA marker, Lane 1: Plasmid DNA, Lane 2: Plasmid DNA linearized by restriction enzyme digestion, Lane 3: Negative control for endpoint PCR and Lane 4: Amplicon from endpoint PCR.

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