SPINeasy DNA Pro Kit for Soil, 50 PREPS

The SPINeasy DNA Pro Kit for Soil has been carefully designed for the isolation of pure microbiome genomic DNA from challenging soil types, including those with low biomass or that are highly contaminated. Soil samples are complex environments characterized by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components, which often prove to be challenging for downstream molecular analysis. The SPINeasy DNA Pro Kit for Soil effectively lyses microbial populations, including bacteria, fungi, viruses, and protists. The kit provides similar yields to that of our highly cited FastDNA™ SPIN Kit, but with improved purity and reduced processing time. Isolated DNA products showed no inhibition in PCR and were immediately ready to be used for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps. Performance Data The SPINeasy DNA Pro Kit for Soil has been developed to accommodate a wide range of soil samples. The kit provides optimal yields, A260/A280, and A260/A230 ratios of DNA using fresh or frozen samples. It displays an exceptional ability to extract DNA from low biomass samples compared to competitor kits (Figure 1, DNA quality). Figure 1. Soil samples with various biomass/ contaminant content (250 mg each) were processed with SPINeasy DNA Pro Kit for Soil and compared to a competitor kit. The DNA yield and purity (A260/A280 and A260/A230 ratio) were assessed using a spectrophotometer. Each point of the plot represents a single extraction. The horizontal bars indicate the median value. DNA integrity was assessed using a 1% agarose gel. For low biomass samples, a similar proportion of DNA eluates were loaded to compare the performance of SPINeasy DNA Pro Kit for Soil and competitor kit (Q pro).

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