SPINeasy Plasmid Miniprep Kit, 50 PREPS

SPINeasy Plasmid Miniprep Kit is a silica membrane spin column kit that enables the purification of up to 20 µg of plasmid DNA from 1 – 5 mL of transformed bacterial cultures. High-purity plasmid DNA can be obtained within 25 minutes through a simple purification process, immediately ready for routine molecular biology applications including cloning, restriction digestion, PCR, and in-vitro transcription. Performance Data Agarose gel electrophoresis, yield, A260/A280 , and A260/A230 ratios of plasmids extracted using SPINeasy Plasmid Miniprep Kit. Plasmids pWPI and pcDNA6 were isolated from 3 mL DH5α cultures harboring these plasmids. M: DNA MarkerLane 1: pWPI plasmidLane 2: pWPI plasmid linearized by restriction digestLane 3: pcDNA6 plasmidLane 4: pcDNA6 plasmid linearized by restriction digest Vector Backbone Plasmid (bp) Extraction results Yield(µg) A(260/280) A(260/230) pWPI 11,103 14.36 1.89 2.18 pcDNA6 5,149 11.24 1.86 2.19

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