Tris-Borate-EDTA buffer, 1 X 20 pkg (PPB009-20PAK)

Tris-Borate-EDTA (TBE) buffer is a commonly used running buffer for nucleic gel electrophoresis with a pH range from 8.1-8.5 (25 °C). TBE buffer has a higher buffer capacity than TAE buffer and has a higher resolution for DNA fragments <1500 bp. Borate in TBE buffer acts as an enzyme inhibitor. Suitable as a running and gel preparation buffer in: DNA agarose gel electrophoresis (fragment <1500 bp) Native and non-denaturing RNA agarose gel electrophoresis (fragment <1500 bp) Denaturing gradient gel electrophoresis Northern and Southern blotting DNA automated sequencing gel Pulsed-field gel electrophoresis (PFGE) (applied voltages of less than 5 V/cm are recommended for maximum resolution) For additional information on our range of Biochemicals, please complete this form. Ready-to-use buffer No measuring and pH adjusting needed Save time and minimize effort Minimize chemical exposure to toxic chemicals Biological tests: free of DNase, RNase, Protease, and Nickase Chemical tests: Iron ≤10 ppm, lead ≤5 ppm Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134). Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use. Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 89mM Tris-Borate, 2mM EDTA, pH 8.3 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in electrophoresis. Recommended temperature for dissolution 5 °C to 40 °C.

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