Trizma® base, 1 X 5 kg (T4661-5KG)

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. Tris base has been used: •As a component of lysis buffer for cell disruption •In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis) •In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC •In capillary electrochromatography and UV analysis of tocopherols and tocotrienols The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Not pyrogen tested. Certificates of analysis are available with lot specific information.

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