Tryptic Soy Broth (TSB) 1000mL Bottle, 1/Pk

This product provides 1 Liter (1000mL) of sterile Tryptic Soy Broth (TSB) in a single bottle. As the most widely used general-purpose medium in microbiology, this bulk volume is essential for aseptic process validation (media fills), large-scale sterility testing, and high-volume culture preparation.

The "Media Fill" Standard: The primary application for 1000mL TSB bottles is Aseptic Process Simulation (APS), often called a "media fill test."

1. The Simulation: Pharmaceutical manufacturers must prove that their filling lines (used for injectables or vaccines) are sterile. Instead of filling vials with expensive drugs, they run this TSB through the machine.

2. The Test: The machine fills hundreds or thousands of vials with the broth. These vials are then incubated.

3. The Result: Because TSB supports such a wide range of bacteria, any contamination in the machine or the environment will cause the broth in the vials to turn cloudy. If the TSB remains clear, the aseptic process is validated.

Bulk Utility: Beyond validation, the 1L format serves as a centralized stock for the lab:

• Large Device Testing: Sufficient volume to fully immerse large medical devices (e.g., catheters, surgical kits, or implants) for sterility testing according to USP <71>.

• Lab-Scale Fermentation: growing large amounts of bacterial biomass for protein extraction or research purposes.

• Dispensing: Cost-effective way to fill custom volumes into smaller tubes or flasks within a biosafety cabinet.

Key Specifications:

• Soybean-Casein Digest: The formulation meets USP, EP, and JP harmonized standards for sterility testing.

• Broad Spectrum: Promotes the growth of aerobic and facultative anaerobic bacteria, as well as fungi (yeasts and molds).

• Clarity: The medium is clear (light amber), ensuring that even faint turbidity (bacterial growth) is visible to the naked eye.

Common Applications:

• Pharma Validation: Media fill trials for aseptic filling lines.

• Sterility Testing: Immersion of bulky medical devices.

• Biomass Production: Growing large cultures for research.

• Bioburden Testing: Testing raw materials with high-volume requirements.

Key Features:

• Bulk Volume: 1000mL capacity for large-scale use.

• Regulatory Compliant: Meets USP <71> standards.

• Universal Growth: Supports bacteria, yeast, and mold.

• Visual Clarity: Easy detection of contamination.

• Format: Single sterile 1000mL screw-cap bottle.

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