Tryptic Soy Broth (TSB) 500mL Bottle, 1/Pk

This product offers 500mL of Tryptic Soy Broth (TSB) in a sterile, ready-to-use bottle. As the standard "Soybean-Casein Digest Medium," this bulk format is a critical consumable for laboratories performing media fill simulations, sterility testing, and large-scale culture maintenance.

The "Mid-Size" Bulk Solution: The 500mL bottle strikes a balance between small tubes and massive 1-liter vessels, making it the preferred size for specific laboratory workflows:

1. Media Fills (Aseptic Validation): Used to validate smaller-scale aseptic filling lines or compounding processes. The liquid is run through the equipment to simulate the filling of vials; any turbidity after incubation signals a breach in sterility.

2. Sterility Testing (Immersion): The 500mL volume allows for the direct immersion of medium-sized medical devices, tubing sets, or surgical instruments that are too large for test tubes but do not require a full liter of media.

3. Stock Media: Serves as a convenient reservoir for dispensing into smaller aliquots inside a biosafety cabinet, reducing the frequency of opening new bottles compared to smaller formats.

Universal Growth Capability: TSB is renowned for its ability to support the growth of a vast array of microorganisms:

• Nutrient Rich: The digest of casein and soy peptones provides the nitrogen, vitamins, and carbon needed for rapid growth.

• Non-Selective: It promotes the growth of aerobic bacteria, facultative anaerobes, and fungi (yeasts and molds), making it the primary choice for detecting general contamination.

Common Applications:

• Pharma Compounding: Validation of aseptic technique (fingertip testing/media fills) in hospital pharmacies (USP <797>).

• Bioburden Testing: Microbial limits testing of non-sterile raw materials.

• Research: Growing bacterial biomass for DNA extraction or protein purification.

• Enrichment: Reviving stressed organisms from food or environmental samples.

Key Features:

• Versatile Volume: 500mL fits many standard validation protocols.

• Broad Spectrum: Supports bacteria, yeast, and molds.

• Regulatory Compliant: Meets USP <71> standards for sterility testing.

• Visual Clarity: Clear amber liquid ensures easy detection of microbial growth (turbidity).

• Format: Single sterile 500mL screw-cap bottle.

Read Less